Recombinant Human CUGBP Elav-like family member 1 (CELF1)

Overview

Recombinant Human CUGBP Elav-like family member 1 (CELF1) is a recombinant protein preparation from Homo sapiens (Human) designed for use in assay development, binding studies, and functional characterization. Key attributes such as expression system, expressed region, and affinity tag(s) help researchers match the reagent to specific experimental readouts.

Key elements and design rationale

• Expression system: E.coli expression is commonly used for rapid, scalable production. For targets that require glycosylation or other post-translational modifications, consider how a prokaryotic system may affect folding or activity.
• Expression region: The expressed fragment (1-486aa) focuses the reagent on a defined domain/segment, which can influence binding interfaces and epitope availability.
• Tag(s)/format: His tags can support purification and detection in pull-down or binding assays; confirm that the tag position does not interfere with the interaction of interest.
• Purity: ≥90% (SDS-PAGE) provides a quick checkpoint for reagent quality in downstream analytical workflows.
• Form: Supplied as Liquid or Lyophilized powder; select the format that best fits your lab’s handling and aliquoting preferences.

Recombinant design choices (expression host, fragment boundaries, and tag configuration) help balance yield, solubility, and assay compatibility. Choose conditions and controls that match the recombinant format to your experimental question.

Biological background

CELF1 has been reported to be involved in Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal. Also involved in the regulation of axon outgrowth and synaptogenesis. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.. When interpreting results, consider species context, domain architecture, and whether the recombinant format represents full-length or a defined region.

Research relevance and current trends

• Mapping synaptic or sensory protein interactions using recombinant domains and binding assays.
• Integrating protein-level readouts with transcriptomics for multi-omic interpretation in neural models.

Common research applications

• Binding and interaction assays: quantify partner binding and rank conditions using plate-based formats or biophysical methods (SPR/BLI).
• Cell-based functional studies: evaluate dose–response and time-course effects in relevant cell systems when the target acts extracellularly or through receptor engagement.
• Assay development: use as a standard, spike-in control, or positive control where consistent specifications are required.

Interpretation typically relies on relative comparisons (treated vs control, mutant vs wild-type, or dose/time series) using consistent sample handling and appropriate normalization.

Notes for experimental interpretation

• Post-translational modifications: expression system can affect glycosylation and processing; interpret differences cautiously when comparing to native protein.
• Isoforms and domains: expressed regions may not capture all isoform-specific features; match fragment boundaries to your assay’s binding site.
• Controls: include blank matrix controls, tag-only controls (where relevant), and orthogonal readouts (e.g., WB/qPCR/ELISA) to support interpretation.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.