| Field | Specification |
|---|---|
| Activity | |
| Alternative Names | NGAL, Lipocalin-2, Oncogene 24p3, Siderocalin LCN2, p25 |
| Biological Activity | |
| Endotoxin Level | |
| Expression System | |
| Form | Lyophilized powder |
| Function | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration.
Product Description
E. coli ExpressionRecombinant Human Neutrophil gelatinase-associated lipocalin protein (LCN2) (Active) is a recombinant protein expressed in E. coli and purified to >95% as determined by SDS-PAGE. as determined by SDS-PAGE. Supplied as Lyophilized powder, tag-free. For research use only.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Protein Specifications
| Molecular Weight | 20.5 kDa |
|---|---|
| Expression Region | 21-198aa |
| Expression System | E. coli |
| Tag | Tag-Free |
| Purity | >95% as determined by SDS-PAGE. |
| Form | Lyophilized powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered PBS, pH 7.4, with 0.05 % Tween-20 |
| Endotoxin Level | Less than 1.0 EU/μg as determined by LAL method. |
| UniProt Accession | P80188 |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Functional Activity
Biologically Validated
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of ＞ 2.0 × 106 IU/mg.
Safety & Handling
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This recombinant protein is expressed in E. coli, a widely used system for producing soluble, biologically active proteins at scale. The E. coli expression platform enables efficient folding and high yields, suitable for functional and binding assays.
This protein is supplied as Tag-Free and has been purified to >95% as determined by SDS-PAGE. as determined by SDS-PAGE. High purity minimizes interference in downstream assays, particularly in cell-based bioactivity studies and ELISA development.
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1–1.0 mg/mL. For long-term storage, add 5–50% glycerol and store in aliquots at -20°C or -80°C to avoid repeated freeze-thaw cycles. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Yes. Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of ＞ 2.0 × 106 IU/mg.
This recombinant protein is suitable for a range of in vitro research applications including binding assays, protein–protein interaction studies, cell proliferation/bioactivity assays, ELISA standard curve calibration, and assay development. Confirm suitability for your specific assay conditions with the product datasheet.
We offer flexible ordering options including bulk quantities, custom formulations, and endotoxin removal services for many of our recombinant proteins. Lead times and minimum order quantities vary by product. For custom conjugation, specialized buffer formulations, or volume pricing, please contact our support team. All customization requests are handled in partnership with our manufacturing suppliers and are subject to feasibility review.
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