| Field | Specification |
|---|---|
| Target | |
| Alternative names | 60S acidic ribosomal protein P0, Ribosomal Phosphoprotein P0, L10E, RPLP0, Ribosomal Protein Large P0, RPP0, P0, PRLP0, MGC88175, MGC111226. |
| Species | |
| Applications | |
| Expression system | |
| Source | Sf9 insect cells. |
| Purity | |
| Cellular localization | |
| Formulation | |
| Form | Sterile Filtered clear solution. |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Recombinant Human Ribosomal Phosphoprotein P0 is supplied as a recombinant protein for in vitro research use.
Background
The ribosomal phosphoproteins, also called P protein antigens, are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa, P1 (19 kDa) and P2 (17 kDa). RPLP0 is a ribosomal protein that is a component of the 60S subunit. RPLP0 belongs to the L10P family of ribosomal proteins. RPLP0 is a neutral phosphoprotein having a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 & P2. The P0 protein interacts with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPLP0 scattered throughout the genome. Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.
Product format
Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.
Activity & assay information
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Immunological functions: 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels. - Coating concentration: 0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
What is the purity of Recombinant Human Ribosomal Phosphoprotein P0 (Human)?
What buffer / formulation is this protein supplied in?
How should Recombinant Human Ribosomal Phosphoprotein P0 (Human) be stored?
What expression system was used to produce this protein?
Is this protein approved for clinical or in vitro diagnostic use?
Can I request a custom size, tag variant, or formulation?
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
