Recombinant Human Small ribosomal subunit protein uS2 (RPSA)

Overview

This Recombinant Protein provides recombinant RPSA from Homo sapiens (Human), produced in E.coli (region 2-295aa). It is commonly used as a defined reagent for assay development, binding studies, and mechanistic research (RUO).

Key elements and design rationale

• Region: 2-295aa (domain boundaries can affect binding/activity readouts).
• Expression host: E.coli (may differ from native PTMs/processing).
• Tag(s): His, Myc (supports purification/detection; consider tag effects in controls).

Biological background

Also reported as 37 kDa laminin receptor precursor (37LRP) (37/67 kDa laminin receptor) (LRP/LR) (67 kDa laminin receptor) (67LR) (Colon carcinoma laminin-binding protein) (Laminin receptor 1) (LamR) (Laminin-binding protein precursor p40) (LBP/p40) (Multidrug resistance-associated protein MGr1-Ag) (NEM/1CHD4) (Small ribosomal subunit protein uS2) (LAMBR) (LAMR1). Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.

Research relevance and current trends

• Quantitative mapping of ligand/receptor signaling to downstream phospho- and transcriptional programs.
• Use of recombinant standards to improve assay calibration and cross-study comparability.

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.

Common research applications

• Standard curve or spike-in reference for quantitative assays involving RPSA
• Binding interaction studies (e.g., SPR/BLI or plate-based binding formats)
• Cell-based stimulation studies with downstream marker readouts (conceptual)

Notes for experimental interpretation

• Recombinant constructs may not capture all native isoforms or PTMs.
• Consider tag- or host-related effects when interpreting binding or activity.
• Use appropriate blanks and matrix/control concepts to separate signal from background.

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