| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Alternative Names | Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA); partial (Active); Brain Ig-like molecule with tyrosine-based activation motifs (Bit) (CD172 antigen-like family member A) (Inhibitory receptor SHPS-1) (Macrophage fusion receptor) (MyD-1 antigen) (Signal-regulatory protein alpha-1) (Sirp-alpha-1) (Signal-regulatory protein alpha-2) (Sirp-alpha-2) (Signal-regulatory protein alpha-3) (Sirp-alpha-3) (p84) (CD_antigen: CD172a) (SHP substrate 1) (SHPS-1) (BIT) (MFR) (MYD1) (PTPNS1) (SHPS1) (SIRP) |
| Biological Activity | |
| Conjugate | |
| Endotoxin Level | |
| Expression System | |
| Form | Lyophilized powder |
| Molecular Weight | |
| Product Type | |
| Protein Length | |
| Purity | |
| Reconstitution | |
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| Target | |
| UniProt # |
Overview
Recombinant Human Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA), partial (Active) is a recombinant protein reagent derived from Homo sapiens (Human) and produced in Mammalian cell. It is commonly used to support Cancer research by enabling enzyme activity assays, kinetics/structure–function studies and inhibitor or substrate screening in controlled in vitro settings.
Key elements and design rationale
- Expressed region: 31-370aa. Region selection can focus on functional domains, improve solubility, or isolate interaction surfaces for targeted studies.
- Expression system: Mammalian cell. Expression host can influence folding and the presence/absence of post-translational modifications.
- Tag / fusion: C-terminal 6xHis-Myc-tagged. Tags can support purification and detection; evaluate potential tag effects when studying sensitive interactions.
- Molecular weight (reported): 41.8 kDa. Apparent size may vary with tags, processing, and gel conditions.
When comparing results across batches or platforms, interpret signals in the context of construct design (region, tags) and expression host, especially for modification-dependent interactions.
Biological background
The gene commonly associated with this target is SIRPA. SIRPA refers to a protein target that is studied across multiple biological contexts; annotations and nomenclature can vary by species and isoform. This product corresponds to the Homo sapiens (Human) sequence context, which can be important when comparing homologs or orthologs across model systems. For curated functional annotations, domains, and sequence features, consult primary databases (e.g., UniProt/NCBI) and the recent literature for the specific organism and isoform.
Research relevance and current trends
- Mapping pathway dependencies and signaling networks that drive tumor growth and drug resistance.
- Developing and benchmarking biomarker assays (e.g., immunoassays or binding reagents) for candidate targets.
- Characterizing protein variants, domains, or interaction partners relevant to targeted therapeutics and precision oncology.
Relevance: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Common research applications
- Enzyme activity assays and kinetics measurements with defined substrates/cofactors.
- Inhibitor, activator, or substrate screening in biochemical assay formats.
- Structure–function analysis to interpret how sequence changes impact catalytic performance.
In quantitative assay development, changes in binding or activity readouts are typically interpreted relative to appropriate negative/positive controls and, where possible, orthogonal assay formats that support the same conclusion.
Notes for experimental interpretation
- Recombinant constructs may represent a defined region (domain) rather than the full-length protein; interpret results in the context of the expressed region.
- Tag or fusion elements can aid purification and detection but may influence binding surfaces or oligomerization; consider tag controls when relevant.
- Species and isoform differences can affect interaction partners and post-translational modifications; align experimental controls to the intended biological context.
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
