| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Alternative Names | MuRantes, Small-inducible cytokine A5, T-cell-specific protein RANTES |
| Biological Activity | |
| Endotoxin Level | |
| Expression System | |
| Form | Lyophilized powder |
| Function | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5.
Product Description
E. coli ExpressionRecombinant Mouse C-C motif chemokine 5 protein (Ccl5) (Active) is a recombinant protein expressed in E. coli and purified to >97% as determined by SDS-PAGE. as determined by SDS-PAGE. Supplied as Lyophilized powder, tag-free. For research use only.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Protein Specifications
| Molecular Weight | 7.9 kDa |
|---|---|
| Expression Region | 24-91aa |
| Expression System | E. coli |
| Tag | Tag-Free |
| Purity | >97% as determined by SDS-PAGE. |
| Form | Lyophilized powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA |
| Endotoxin Level | Less than 1.0 EU/μg as determined by LAL method. |
| UniProt Accession | P30882 |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Functional Activity
Biologically Validated
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T lymphocytes is in a concentration range of 1.0-10 ng/ml.
Safety & Handling
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Browse additional recombinant proteins, antibodies, and ELISA kits available through BioHippo to support your research workflow.
This recombinant protein is expressed in E. coli, a widely used system for producing soluble, biologically active proteins at scale. The E. coli expression platform enables efficient folding and high yields, suitable for functional and binding assays.
This protein is supplied as Tag-Free and has been purified to >97% as determined by SDS-PAGE. as determined by SDS-PAGE. High purity minimizes interference in downstream assays, particularly in cell-based bioactivity studies and ELISA development.
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1–1.0 mg/mL. For long-term storage, add 5–50% glycerol and store in aliquots at -20°C or -80°C to avoid repeated freeze-thaw cycles. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Yes. Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T lymphocytes is in a concentration range of 1.0-10 ng/ml.
This recombinant protein is suitable for a range of in vitro research applications including binding assays, protein–protein interaction studies, cell proliferation/bioactivity assays, ELISA standard curve calibration, and assay development. Confirm suitability for your specific assay conditions with the product datasheet.
We offer flexible ordering options including bulk quantities, custom formulations, and endotoxin removal services for many of our recombinant proteins. Lead times and minimum order quantities vary by product. For custom conjugation, specialized buffer formulations, or volume pricing, please contact our support team. All customization requests are handled in partnership with our manufacturing suppliers and are subject to feasibility review.
