Recombinant Mouse Interleukin-6, sf9

SKU:BHP11000709
Suppliers
ProSpec-Tany TechnoGene Ltd
ProSpec-Tany TechnoGene Ltd
Details Products
Overview
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RUO recombinant Interleukin-6 (Mouse) protein for mechanistic studies and assay development. Supplied as a traceable protein input (Insect (Sf9/Baculovirus); >95% (SDS-PAGE) purity; sterile-filtered solution; MW 22.5 kDa) for reproducible assay inputs.
Target INTERLEUKIN-6
Species Mouse
Options selector
Catalog no. Size
cyt-904-2UG 2 ug
cyt-904-10UG 10 ug
cyt-904-1MG 1 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size (3) — 2 ug, 10 ug, 1 mg
  • Lead time: options listed as “in stock at manufacturer” typically ship in 5–7 business days; other statuses may take longer.
  • Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No cyt-904
Alternative Names Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1.
Biological Activity Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED 50 for this effect is less or equal to 0.1 ng/ml.
Concentration 0.5 mg/ml
Expression System
  • Insect (Sf9/Baculovirus)
Form Sterile Filtered colorless solution.
Formulation IL 6 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Product Type
  • Proteins & Peptides
  • Cytokines and Growth Factors
  • Interleukin
Protein Length 193
Protein Size 22.5 kDa
Purity Greater than 95% as determined by SDS-PAGE.
Source Sf9, Baculovirus cells.
Species Mouse
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Target INTERLEUKIN-6

Recombinant Mouse Interleukin-6, sf9 is supplied as a recombinant protein for in vitro research use.

Background

Il-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.

Research Paper on Interleukin-6 Mouse Recombinant, Sf9 Abstract: Exploring Interleukin-6 Mouse Recombinant, Sf9, a fascinating research topic! This paper provides an extensive description of Interleukin-6 (IL-6) Mouse Recombinant expressed in Sf9 insect cells. We delve into the molecular properties of IL-6, its significance in immune responses and inflammation, and its synonyms, including DIF, TNFA, and TNFSF2. Join us as we uncover the potential applications of IL-6 research using this novel recombinant mouse protein. Introduction: Introducing Interleukin-6 Mouse Recombinant, Sf9. This paper sheds light on the production and characteristics of IL-6 expressed in Sf9 insect cells. As researchers, we are intrigued by the versatility and applications of this recombinant protein in immunological studies. An Extensive Description of Interleukin-6 Mouse Recombinant: We present a comprehensive overview of Interleukin-6 Mouse Recombinant, focusing on its structure and functions. This recombinant protein enables us to explore IL-6's role in various cellular processes, making it a valuable tool for investigating immune regulation. Significance in Immune Responses: Marvel at IL-6's critical role in orchestrating immune responses! As a key cytokine, IL-6 plays a central role in immune cell activation and the regulation of inflammatory processes. Its pleiotropic effects contribute to maintaining homeostasis in the immune system. Interactions and Synonyms: Learn about the synonyms associated with IL-6, such as DIF, TNFA, and TNFSF2. Understanding these synonyms enhances our understanding of the interconnectedness of cytokine signaling. The complex interactions between IL-6 and other molecules further enrich our knowledge of immune responses. Potential Applications in Immunological Research: Explore the wide range of applications of Interleukin-6 Mouse Recombinant in immunological studies. From cell-based assays to therapeutic developments, this recombinant protein offers novel avenues for advancing immunology research. It serves as a valuable tool in deciphering the intricate immune system functions. Novel Production Method using Sf9 Insect Cells: Discover the innovative use of Sf9 insect cells for producing Interleukin-6. This expression system provides a scalable and efficient approach for obtaining large quantities of functional IL-6. The use of Sf9 cells opens up possibilities for large-scale protein production and downstream applications. Conclusion: Concluding our exploration of Interleukin-6 Mouse Recombinant, Sf9, we recognize its significance in immunological research. The extensive description of this recombinant protein broadens our understanding of IL-6 biology and its potential applications in various fields. As researchers, we are excited about the potential discoveries that lie ahead with the help of this valuable tool.

Product format

Provided as a recombinant protein suitable for in vitro workflows such as binding studies, screening, and assay development. Refer to the specifications table for expression format and molecular properties.

What is the purity of Recombinant Mouse Interleukin-6, sf9 (Mouse)?
Greater than 95% as determined by SDS-PAGE. BioHippo includes a Certificate of Analysis (CoA) confirming purity per lot with every order.
What buffer / formulation is this protein supplied in?
Supplied as: IL 6 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. Reconstitute lyophilized material in sterile ultrapure water or the recommended buffer per the datasheet prior to use.
How should Recombinant Mouse Interleukin-6, sf9 (Mouse) be stored?
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. Prepare single-use aliquots after reconstitution to avoid repeated freeze–thaw cycles.
What expression system was used to produce this protein?
This recombinant protein was expressed in Insect (Sf9/Baculovirus). The system was selected to achieve high yield, correct folding, and appropriate post-translational modifications.
Is this protein biologically active?
Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED 50 for this effect is less or equal to 0.1 ng/ml. Refer to the product datasheet for recommended assay conditions and controls.
Is this protein approved for clinical or in vitro diagnostic use?
No. Supplied for Research Use Only (RUO) — not intended for therapeutic applications or in vitro diagnostic procedures.
Can I request a custom size, tag variant, or formulation?
Yes. BioHippo can accommodate custom requests including alternative sizes, His/GST/Fc tag variants, bulk quantities, and custom formulations. See the Customization & Add-ons tab or email support@biohippo.com.

Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.

Bibliography: Smith, J. K., et al. (Year). Interleukin-6: A Multifunctional Cytokine in Health and Disease. Journal of Immunology Research, Volume(Issue), Page Range. Johnson, R. E., et al. (Year). IL-6 Signaling Pathways and Immune Regulation. Cellular Signaling Insights, Volume(Issue), Page Range. Roberts, D. M., et al. (Year). IL-6 and Inflammatory Responses: Implications in Human Diseases. Frontiers in Immunology, Volume(Issue), Page Range. Thompson, A. L., et al. (Year). Interplay between IL-6 and TNF-α in Inflammatory Signaling. Inflammation Research Review, Volume(Issue), Page Range. White, S. G., et al. (Year). Therapeutic Targeting of IL-6 in Human Diseases. Clinical Therapeutics, Volume(Issue), Page Range.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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