| Field | Specification |
|---|---|
| Mfr No | |
| Biological Activity | |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Source | Escherichia coli |
| Storage |
Scientific Background
Macrophage Inflammatory Protein-1 gamma (MIP-1 gamma), also called MIP-2, belongs to the β (or CC) intercrine family of chemokines. It is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines. This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon Nterminal truncation.
Product Description
E. coli ExpressionRecombinant Macrophage Inflammatory Protein-1 gamma/ CCL9/10 Mouse protein is produced using a validated E. coli expression system and supplied as lyophilized powder for long-term stability. Suitable for use in functional bioassays, ELISA standard curves, receptor binding studies, antibody validation, and related research applications.
Protein Specifications
| Expression System | E. coli |
|---|---|
| Molecular Weight | Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids. |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Endotoxin | Less than 1EU/μg of rMuCCL9/10 as determined by LAL method. |
| Physical Form | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, … |
Functional Activity
Measured by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.002-0.01 μg/mL.
Safety & Handling
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
This protein was produced in E. coli expression system. Expression system selection determines glycosylation profile, folding, and post-translational modifications. For cell-based stimulation assays, verify the expression system matches the glycosylation requirements of your target receptor or pathway.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions. Reconstitute in sterile distilled water or PBS at 100 µg/mL as a standard starting concentration. Allow to dissolve at 4°C for 30 minutes without vortexing. Prepare working aliquots in 0.1% BSA carrier protein and store at −80°C.
This protein has been validated for functional bioactivity: Measured by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.002-0.01 μg/mL.. Optimal working concentrations may vary depending on your cell type, assay format, and culture conditions. Titrate the protein in a dose-response experiment to determine the optimal concentration for your system.
Endotoxin level is Less than 1EU/μg of rMuCCL9/10 as determined by LAL method. as determined by the LAL method. Low endotoxin is critical for cell-based studies because endotoxin activates NF-κB and TLR4 signalling in immune cells, producing artefactual cytokine induction that can completely mask the true biological activity of the recombinant protein.
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. F Upon receipt, immediately store at −80°C. Prepare single-use working aliquots to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 6–12 months at −80°C from the date of receipt.
BioHippo offers flexible sourcing options for qualified research institutions and partners. The following may be available subject to supplier capabilities and order volume.
- Custom quantities: Bulk pricing or non-standard sizes available for high-throughput screening or scale-up projects.
- Custom formulation: Alternative reconstitution buffers or carrier proteins may be accommodated on request.
- Extended QC data: Additional bioactivity assay data, endotoxin reports, or SEC-HPLC purity profiles available on request.
Contact BioHippo customer support to discuss your requirements.
