| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Alternative Names | (Folate hydrolase 1)(Folylpoly-gamma-glutamate carboxypeptidase)(FGCP)(Glutamate carboxypeptidase II)(GCPII)(Membrane glutamate carboxypeptidase)(mGCP)(N-acetylated-alpha-linked acidic dipeptidase I)(NAALADase I)(Prostate-specific membrane antigen homolog)(Pteroylpoly-gamma-glutamate carboxypeptidase) |
| Conjugate | |
| Endotoxin Level | |
| Expression System | |
| Form | Liquid or Lyophilized powder |
| Molecular Weight | |
| Product Type | |
| Protein Length | |
| Purity | |
| Reconstitution | |
| Species | |
| Storage | |
| Target | |
| UniProt # |
Overview
Recombinant Pig Glutamate carboxypeptidase 2 (FOLH1), partial, Biotinylated is a recombinant protein preparation from Sus scrofa (Pig) designed for use in assay development, binding studies, and functional characterization. Key attributes such as expression system, expressed region, and affinity tag(s) help researchers match the reagent to specific experimental readouts.
Key elements and design rationale
- Expression system: E.coli expression is commonly used for rapid, scalable production. For targets that require glycosylation or other post-translational modifications, consider how a prokaryotic system may affect folding or activity.
- Expression region: The expressed fragment (44-751aa) focuses the reagent on a defined domain/segment, which can influence binding interfaces and epitope availability.
- Tag(s)/format: His tags can support purification and detection in pull-down or binding assays; confirm that the tag position does not interfere with the interaction of interest.
- Purity: ≥85% (SDS-PAGE) provides a quick checkpoint for reagent quality in downstream analytical workflows.
- Form: Supplied as Liquid or Lyophilized powder; select the format that best fits your lab’s handling and aliquoting preferences.
Recombinant design choices (expression host, fragment boundaries, and tag configuration) help balance yield, solubility, and assay compatibility. Choose conditions and controls that match the recombinant format to your experimental question.
Biological background
FOLH1 has been reported to be involved in Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. ; Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.. When interpreting results, consider species context, domain architecture, and whether the recombinant format represents full-length or a defined region.
Research relevance and current trends
- Mapping synaptic or sensory protein interactions using recombinant domains and binding assays.
- Integrating protein-level readouts with transcriptomics for multi-omic interpretation in neural models.
Common research applications
- Binding and interaction assays: quantify partner binding and rank conditions using plate-based formats or biophysical methods (SPR/BLI).
- Enzymology: assess catalytic activity and compare substrate preferences or inhibitor effects using appropriate controls.
- Assay development: use as a standard, spike-in control, or positive control where consistent specifications are required.
Interpretation typically relies on relative comparisons (treated vs control, mutant vs wild-type, or dose/time series) using consistent sample handling and appropriate normalization.
Notes for experimental interpretation
- Post-translational modifications: expression system can affect glycosylation and processing; interpret differences cautiously when comparing to native protein.
- Isoforms and domains: expressed regions may not capture all isoform-specific features; match fragment boundaries to your assay’s binding site.
- Controls: include blank matrix controls, tag-only controls (where relevant), and orthogonal readouts (e.g., WB/qPCR/ELISA) to support interpretation.
Can’t Find What You’re Looking For? We can help you source the best match or customize a recombinant protein solution for your study. Options may include species (human/mouse/rat), protein region/domain (full-length vs fragment), tag or label (His/GST/FLAG/biotin/fluorescent), expression system (E. coli/HEK293/insect), purity grade, formulation (buffer, carrier-free, glycerol-free), activity/functional validation (binding or enzymatic assays), endotoxin level (low-endotoxin for cell-based work), mutants/variants (point mutations, isoforms), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
