| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human REM2 recombinant protein (Position: R65-E257) was used as the immunogen for the REM2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
REM2 Antibody / Rad and Gem-like GTP-binding protein 2 is a anti-REM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: REM2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
REM2 is encoded by the REM2 gene on human chromosome 14q23.2. The protein is approximately 36 kilodaltons and shares structural similarity with other RGK family members. It acts as a potent inhibitor of voltage-dependent calcium channels by binding to beta subunits, reducing calcium influx in excitable cells. This activity modulates neuronal excitability and synaptic plasticity, linking REM2 expression to learning and memory processes.
The REM2 antibody detects a 36-38 kilodalton protein by western blot and exhibits cytoplasmic staining in neurons, muscle cells, and other excitable tissues. REM2 expression is dynamically regulated by neuronal activity and signaling pathways involving calcium/calmodulin-dependent kinases and cAMP response element-binding protein (CREB). Overexpression of REM2 suppresses calcium current density, alters actin filament organization, and inhibits dendritic spine formation. Conversely, its downregulation promotes neurite outgrowth and synaptic connectivity.
Beyond neuronal contexts, REM2 contributes to cytoskeletal remodeling and cell proliferation. It interacts with 14-3-3 proteins and signaling kinases, coordinating calcium-dependent gene expression and structural plasticity. Dysregulation of REM2 has been associated with mood disorders, neuropathic pain, and cardiac remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
