| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Renin receptor/ATP6AP2 was used as the immunogen for the Renin receptor antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Renin receptor Antibody / ATP6AP2 is a anti-ATP6AP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER, Lysosome).
Key elements and design rationale
- Target: ATP6AP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, FACS
Biological background
Functionally, ATP6AP2 binds both renin and its precursor prorenin, enhancing angiotensin I generation and activating intracellular signaling pathways independent of angiotensin II. This binding promotes cell proliferation, fibrosis, and inflammation through ERK1/2 and MAPK activation. In the kidney, ATP6AP2 is localized to juxtaglomerular and distal tubular cells, where it regulates renin signaling and fluid balance. As a V-ATPase accessory subunit, it stabilizes proton pump assembly, contributing to lysosomal acidification and endosomal trafficking.
Structurally, ATP6AP2 contains an extracellular renin-binding domain, a single transmembrane segment, and a short cytoplasmic tail that mediates interactions with the V-ATPase complex. It is classified within the V-ATPase assembly factor family, sharing homology with ATP6AP1. Co-localization studies show ATP6AP2 at the plasma membrane, endosomes, and lysosomes, reflecting its multifunctional localization and dual biological roles. Known interaction partners include renin, prorenin, and the V-ATPase V0 domain subunits.
ATP6AP2 is widely expressed in cardiovascular and neural tissues, with developmental expression detected in embryonic heart and brain, suggesting roles in organogenesis. In the central nervous system, ATP6AP2 modulates neuronal development, synaptic transmission, and autophagy regulation. Dysregulation of ATP6AP2 expression or mutation causes X-linked intellectual disability and Parkinsonism with spasticity (ATP6AP2-related disorder). In vascular smooth muscle and cardiac fibroblasts, ATP6AP2 signaling contributes to hypertension, cardiac hypertrophy, and fibrosis through MAPK and TGF-beta activation.
Clinically, elevated ATP6AP2 expression is associated with cardiovascular diseases, diabetic nephropathy, and tumor progression. It influences Wnt and Notch signaling pathways that regulate proliferation and differentiation. Pathway associations include renin-angiotensin signaling, proton transport via V-ATPase, and lysosomal degradation. Tissue-specific studies show high ATP6AP2 activity in renal epithelia and cardiomyocytes, coordinating hormonal and metabolic regulation.
Immunohistochemical staining using Renin receptor antibody reveals membrane and cytoplasmic localization in kidney and cardiac tissues. The Renin receptor antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
