| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human REPS2 recombinant protein (Position: A76-K564) was used as the immunogen for the REPS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
REPS2 Antibody / RalBP1-associated Eps domain-containing protein 2 is a anti-REPS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: REPS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, REPS2 antibody identifies a 639-amino-acid protein containing EH (Eps15 homology) domains that mediate interactions with endocytic machinery proteins including Eps15, RalBP1, and AP-2. REPS2 contributes to the internalization of epidermal growth factor receptor (EGFR) and other tyrosine kinase receptors, influencing receptor recycling and downregulation. It also participates in the regulation of cytoskeletal dynamics and cell adhesion through Ral GTPase-dependent pathways.
The REPS2 gene is located on chromosome Xp22.3 and is expressed in epithelial tissues, neurons, and endocrine glands. In addition to its role in endocytosis, REPS2 modulates insulin signaling, exocytosis, and vesicular trafficking, providing a link between receptor activation and intracellular transport systems.
Pathologically, aberrant REPS2 expression has been implicated in prostate cancer, breast cancer, and neurological disorders. Overexpression promotes cancer cell migration and survival by sustaining receptor tyrosine kinase signaling, while loss of REPS2 can impair receptor internalization and lead to altered growth factor responsiveness. Research using REPS2 antibody supports studies in membrane trafficking, cell signaling, and tumor progression.
REPS2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect endocytic adaptor proteins.
Structurally, RalBP1-associated Eps domain-containing protein 2 contains N-terminal EH domains, central coiled-coil motifs, and proline-rich regions that mediate protein-protein interactions. These domains form scaffolds for complex assembly with signaling and endocytic components. This antibody enables the analysis of REPS2's role in receptor-mediated endocytosis and signaling modulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
