| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human REST recombinant protein (Position: E110-E1097) was used as the immunogen for the REST antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
REST Antibody / RE1-silencing transcription factor is a anti-REST Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: REST
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, REST antibody identifies a 1097-amino-acid nuclear protein that suppresses transcription by forming repressive chromatin structures. REST recognizes RE1 sites present in the promoters of neuronal genes and recruits corepressors such as CoREST, mSin3, and histone deacetylases (HDAC1/2). These interactions promote chromatin condensation and transcriptional silencing, preventing inappropriate neuronal gene expression in non-neuronal tissues.
The REST gene is located on chromosome 4q12 and encodes a multi-zinc finger transcription factor with eight C2H2 zinc finger motifs that mediate sequence-specific DNA binding. REST acts as a master regulator of neurogenesis, ensuring proper timing of neuronal differentiation. During development, REST levels decline in differentiating neurons, allowing the expression of genes required for synaptic function, ion channel activity, and neurotransmitter release.
In addition to developmental regulation, REST participates in neural plasticity, stem cell maintenance, and stress response pathways. Dysregulation of REST has been linked to neurological disorders, including epilepsy, Huntington's disease, and Alzheimer's disease. Elevated REST expression in non-neuronal cancers such as medulloblastoma and neuroblastoma suggests a role in tumorigenesis through repression of differentiation pathways.
REST antibody is widely used in neuroscience, epigenetics, and cancer research. It is suitable for chromatin immunoprecipitation (ChIP), immunoblotting, and immunofluorescence to study REST localization, DNA binding, and interaction networks. This antibody aids in exploring transcriptional silencing mechanisms and neurodevelopmental regulation. In oncology, REST serves as a biomarker of altered differentiation and epigenetic reprogramming.
Structurally, REST contains an N-terminal repression domain that recruits mSin3-HDAC complexes and a C-terminal domain that binds CoREST and associated enzymes such as LSD1. REST-mediated repression involves coordinated histone deacetylation and demethylation, establishing a transcriptionally repressive chromatin environment.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
