| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RGL3 recombinant protein (Position: Q49-D622) was used as the immunogen for the RGL3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RGL3 Antibody / Ral guanine nucleotide dissociation stimulator-like 3 is a anti-RGL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: RGL3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
RGL3 antibody identifies a multifunctional signaling adaptor that localizes primarily to the cytoplasm and cell membrane, where it interacts with active GTP-bound Ras. Through its Ras-binding domain (RBD) and pleckstrin homology (PH) domain, RGL3 serves as a molecular bridge linking Ras activation to downstream Ral-mediated signaling. This pathway plays critical roles in exocytosis, endocytosis, and integrin-dependent cell migration. Structural analyses reveal that RGL3 possesses distinct domains for Ras and phospholipid interactions, allowing it to coordinate spatial and temporal aspects of Ral signaling.
Functionally, RGL3 participates in pathways controlling cytoskeletal remodeling and membrane trafficking. Activated RGL3 promotes the assembly of Ral effector complexes involved in vesicle tethering and actin reorganization. In epithelial cells, it contributes to junction formation and cell polarity maintenance. The Ral signaling axis, modulated by RGL3, influences diverse cellular functions including autophagy, exosome release, and oncogenic transformation.
RGL3 is transcriptionally regulated by growth factors and mitogenic stimuli, positioning it as an important node in the Ras-MAPK signaling network. Dysregulation of RGL3 expression or activity has been linked to cancer progression, metastasis, and neurological disorders. Overactivation of the Ral GTPase pathway through RGL3 can enhance tumor cell migration and invasion, particularly in pancreatic and lung cancers. Conversely, loss of RGL3 function can impair vesicular transport and disrupt cell adhesion dynamics.
At the pathway level, RGL3 participates in Ras and Ral GTPase signaling cascades, which intersect with PI3K and MAPK pathways to control cell survival and differentiation. Evolutionarily, RGL3 belongs to the RalGDS family of guanine nucleotide exchange factors, sharing homology with RGL1, RGL2, and RalGPS proteins. The protein's regulatory domains ensure precise spatiotemporal activation of Ral GTPases in response to external stimuli.
Immunohistochemical analysis using RGL3 antibody shows cytoplasmic and membrane localization in epithelial and neuronal cells, consistent with its role in Ras-dependent signaling. RGL3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
