| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RHBG recombinant protein (Position: R7-L394) was used as the immunogen for the RHBG antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RHBG Antibody / Ammonium transporter Rh type B is a anti-RHBG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: RHBG
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, RHBG antibody identifies a 458-amino-acid multi-pass transmembrane protein localized to the basolateral membrane of epithelial cells in renal collecting ducts and hepatocytes. RHBG facilitates the diffusion of ammonia across cell membranes, coupling nitrogen excretion with pH homeostasis. It functions as part of the ammonia recycling pathway that adjusts renal acid secretion according to metabolic conditions. RHBG complements the activity of the related transporter RHCG, which operates on the apical membrane of renal tubular cells to mediate ammonia excretion into urine.
The RHBG gene is located on chromosome 1q21.3 and encodes a protein containing 12 predicted transmembrane domains typical of ammonium transporters. Structural studies indicate that RHBG operates as a gas channel rather than an ion exchanger, selectively permeable to ammonia. Its expression is regulated by systemic acid-base status, hormonal control, and dietary nitrogen intake. In the liver, RHBG contributes to ammonia detoxification by facilitating its diffusion into hepatocytes for conversion to urea via the urea cycle.
Disruption of RHBG function leads to impaired ammonia transport and metabolic acidosis. Altered expression has been observed in chronic kidney disease and hepatic encephalopathy, conditions characterized by disordered nitrogen metabolism. In addition to its renal and hepatic roles, RHBG is expressed in brain, skin, and reproductive tissues, where it contributes to local pH and nitrogen regulation. Its regulation by hypoxia-inducible factors (HIFs) and glucocorticoids underscores its involvement in physiological adaptation to metabolic stress.
RHBG antibody is widely used in renal physiology, hepatology, and membrane transport research. It is suitable for immunoblotting, immunohistochemistry, and confocal microscopy to analyze RHBG expression and localization. In kidney research, this antibody helps distinguish basolateral ammonia transport from apical excretion mechanisms. Its detection supports studies on acid-base homeostasis, ammonia metabolism, and renal tubular function. RHBG also serves as a biomarker for nitrogen-handling capacity in metabolic and toxicological studies.
Structurally, RHBG belongs to the Amt/Mep/Rh superfamily of ammonia transporters. It forms homotrimers in the membrane, each subunit containing a hydrophobic pore that selectively channels ammonia. Post-translational modifications such as glycosylation influence its membrane stability and transport efficiency.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
