| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RIG recombinant protein (Position: M1-L110) was used as the immunogen for the RIG antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RIG Antibody / Protein regulated in glioma is a anti-RIG Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RIG
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
RIG localizes predominantly to the nucleus and may function as a DNA- or RNA-binding protein that modulates transcriptional output under conditions of metabolic or proliferative stress. Its small size and sequence composition suggest that it acts as an auxiliary nuclear factor rather than a canonical enzyme. Expression analyses from glioma and astrocytoma models indicate that RIG levels can fluctuate during tumor progression, possibly reflecting changes in transcriptional activity, cell cycle control, or differentiation signaling. Overexpression of RIG in some glioma-derived lines has been linked to enhanced proliferation and resistance to apoptotic stimuli, whereas reduced expression can coincide with altered cell morphology or loss of growth capacity.
The RIG gene encodes a compact protein that may influence chromatin accessibility or RNA processing. It is expressed in several tissues but shows highest abundance in neural and glial populations. Its amino acid sequence contains motifs compatible with nuclear localization and RNA recognition, supporting a role in coordinating transcriptional output with cellular stress or growth cues. Although the detailed mechanisms remain under investigation, RIG is thought to act as a nuclear modulator of gene expression relevant to glioma pathogenesis.
At the cellular level, RIG expression has been reported to respond to external stimuli such as serum withdrawal, oxidative stress, and exposure to differentiation agents. These findings suggest that RIG may help cells adapt transcriptionally to environmental or developmental cues. In glioma models, its upregulation is often associated with actively dividing cells, consistent with a role in sustaining nuclear RNA metabolism and growth-associated gene expression programs.
RIG antibody provides a specific reagent for detecting this 12 kDa nuclear protein in cultured cells and tissue extracts. It supports research focused on glioma biology, transcriptional regulation, and nuclear protein function. The antibody can aid in identifying RIG expression patterns in tumor versus normal brain tissue and in exploring nuclear pathways involved in glial differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
