| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RING1 recombinant protein (Position: N6-K387) was used as the immunogen for the RING1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RING1 Antibody / Polycomb complex protein RING1 is a anti-RING1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: RING1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, RING1 antibody identifies a 406-amino-acid nuclear enzyme containing a RING finger domain that mediates E3 ubiquitin ligase activity. RING1 monoubiquitinates histone H2A at lysine 119, a hallmark modification that represses transcription of developmental genes. It forms a heterodimer with RING1B or BMI1, together constituting the catalytic core of PRC1. This activity ensures stable repression of genes controlling cell fate and pluripotency.
The RING1 gene is located on chromosome 6p22.3 and is broadly expressed, with high levels in embryonic and stem cell populations. RING1 functions as an epigenetic regulator, linking chromatin compaction to long-term gene silencing. Its activity is modulated by PRC2-mediated H3K27 methylation, which recruits PRC1 to specific genomic loci.
Pathologically, deregulated RING1 expression contributes to developmental abnormalities and cancer. Overexpression of PRC1 components, including RING1, enhances chromatin repression and promotes oncogenic transformation. Conversely, loss of RING1 impairs differentiation and disrupts gene silencing. Research using RING1 antibody aids studies in epigenetic regulation, chromatin structure, and stem cell biology.
RING1 antibody is suitable for western blotting, immunoprecipitation, and chromatin immunoprecipitation to detect PRC1 complex components and histone modifications.
Structurally, RING1 possesses an N-terminal RING finger motif coordinating two zinc ions essential for ubiquitin transfer. It interacts with E2 ubiquitin-conjugating enzymes to catalyze substrate modification. This antibody enables analysis of RING1's central role in chromatin remodeling and transcriptional repression through histone ubiquitination.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
