| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human RNF115 was used as the immunogen for the RNF115 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
RNF115 Antibody / Ring finger protein 115 is a anti-RNF115 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R26 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RNF115
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30R26, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
RNF115 antibody is widely applied in cancer research, immunology, and cell biology. Originally identified as BCA2 in breast cancer, RNF115 is implicated in tumor progression and resistance to therapy. As an E3 ligase, it regulates receptor endocytosis, innate immune signaling, and viral restriction pathways. By detecting RNF115, researchers can explore how ubiquitin mediated regulation impacts cancer and immunity.
Applications for RNF115 antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies RNF115 in tumor and immune cell lysates, immunohistochemistry maps expression in cancer biopsies, and immunofluorescence reveals subcellular localization at endosomes. These techniques provide comprehensive coverage of RNF115 biology in experimental and clinical research.
RNF115 interacts with Rab7 and other trafficking proteins to control endosomal sorting. It also modulates innate immune signaling by regulating proteins such as MAVS and STING. Viral proteins can target RNF115 to escape host defense, making it a focus in virology. In breast and other cancers, RNF115 overexpression correlates with proliferation, migration, and poor prognosis. By applying RNF115 antibody, scientists can link ubiquitin signaling to diverse disease processes.
Therapeutic targeting of E3 ligases is an emerging strategy in drug discovery. RNF115 represents a potential target in cancer and infectious disease, where modulating ubiquitin transfer may alter signaling outcomes. The antibody provides a powerful tool for mechanistic studies and translational research exploring these therapeutic avenues.
RNF115 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
