| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ROCK1 was used as the immunogen for the ROCK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ROCK1 Antibody / Rho associated coiled coil containing protein kinase 1 is a anti-ROCK1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32R84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: ROCK1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32R84, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP, FACS
Biological background
Rho associated coiled coil containing protein kinase 1 is activated by binding to the small GTPase RhoA. Research using ROCK1 antibody has shown that upon activation, the kinase phosphorylates substrates such as LIM kinase, myosin light chain phosphatase, and ERM proteins. These phosphorylation events regulate actin filament assembly, stress fiber formation, and focal adhesion dynamics. Through these activities, ROCK1 coordinates changes in cell shape and migration in response to extracellular signals.
Studies with ROCK1 antibody have revealed that the kinase is essential for smooth muscle contraction, axon guidance, and angiogenesis. ROCK1 participates in vascular tone regulation by modulating smooth muscle contractility, while in the nervous system, it influences growth cone dynamics during axonal pathfinding. In endothelial cells, ROCK1 activity supports sprouting angiogenesis and vessel stability. These diverse roles emphasize its broad physiological importance.
Dysregulation of Rho associated coiled coil containing protein kinase 1 contributes to disease. Research using ROCK1 antibody has demonstrated that aberrant activation promotes cancer cell invasion and metastasis by remodeling the actin cytoskeleton. In cardiovascular disease, excessive ROCK1 activity contributes to vascular stiffness and hypertension. In neurological disorders, altered ROCK1 function affects axonal regeneration and contributes to neurodegeneration. These findings highlight ROCK1 as a therapeutic target in multiple pathological contexts.
Pharmacological inhibition of ROCK1 has been explored for therapeutic benefit. Studies with ROCK1 antibody have supported the development of selective inhibitors such as fasudil and ripasudil, which show efficacy in treating pulmonary hypertension, glaucoma, and other conditions involving abnormal ROCK signaling. This underscores the clinical relevance of monitoring ROCK1 expression and activity.
ROCK1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects kinase expression and post-translational modifications, immunohistochemistry demonstrates tissue distribution, and immunofluorescence shows cytoplasmic localization associated with actin fibers. These applications make ROCK1 antibody versatile in cell biology and disease research.
By providing validated ROCK1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
