RORα Reporter Lentivirus

Background

RORα (RAR-related orphan receptor alpha, NR1F1) is a ligand-dependent nuclear receptor that binds ROR response elements (ROREs) in target gene promoters as a monomer. RORα regulates a broad transcriptional program controlling circadian rhythm, lipid and glucose metabolism, inflammation, and cellular development, and it is highly expressed in tissues such as the cerebellum, liver, and immune cells. RORα contributes to the molecular clock and modulates immune cell function, with deficiency selectively reducing release of pro-inflammatory cytokines. Because of these roles, RORα is studied as a target in circadian biology, metabolic disease, and inflammation, and reporter systems based on RORE motifs allow quantitative assessment of its activity.

Product Description & Applications

The RORα Reporter Lentivirus is a transcription-factor reporter system for detecting RORα-mediated transcriptional activity in mammalian cells. The construct uses tandem repeats of the RORE core motif flanked by RORα-specific sequences to preferentially monitor RORα activity, coupled to a minimal promoter that drives a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.

Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding the variability of transient transfection. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on RORα in circadian regulation, metabolism, and inflammation.

About This Product

This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RORa transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.