| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RPL14 recombinant protein (Position: M1-A207) was used as the immunogen for the RPL14 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPL14 Antibody / 60S ribosomal protein L14 is a anti-RPL14 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: RPL14
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
RPL14 is encoded by the RPL14 gene located on human chromosome 3p21.3. The protein is about 23 kilodaltons and localizes predominantly in the cytoplasm and nucleolus. RPL14 integrates into the 60S ribosomal subunit near the peptidyl transferase center, stabilizing interactions between rRNA and ribosomal proteins during translation. Through these roles, RPL14 ensures accuracy and efficiency of protein synthesis.
The RPL14 antibody detects a 23 kilodalton band on western blot and demonstrates both cytoplasmic and nucleolar staining by immunofluorescence. RPL14 expression correlates with proliferative activity, and its upregulation is often seen in rapidly dividing cells or tumors. Inhibition of RPL14 synthesis activates ribosomal stress pathways, leading to p53 stabilization and growth arrest. Mutations or pseudogene-derived transcripts of RPL14 may contribute to tumorigenesis or ribosomopathies characterized by anemia and developmental defects.
Beyond translation, RPL14 participates in extra-ribosomal functions, including regulation of apoptosis and cell cycle progression. Under stress conditions, RPL14 can interact with transcription factors or signaling proteins, modulating gene expression independent of ribosome assembly. Proteomic studies have also detected RPL14 in mitochondrial ribosome fractions, suggesting roles in organelle-specific translation.
Because ribosomal proteins are frequently deregulated in cancer, RPL14 serves as a biomarker for tumor cell proliferation and ribosome biogenesis activity. Elevated expression has been observed in colorectal, breast, and ovarian cancers, correlating with poor prognosis. Conversely, knockdown of RPL14 reduces protein synthesis and inhibits tumor growth.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
