| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RPL24 recombinant protein (Position: M1-R157) was used as the immunogen for the RPL24 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPL24 Antibody / 60S ribosomal protein L24 is a anti-RPL24 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: RPL24
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
Structurally, RPL24 is a 157-amino-acid protein of approximately 18 kilodaltons that forms part of the ribosomal 60S subunit. It is highly conserved across species and interacts with ribosomal RNA and neighboring proteins to stabilize the ribosomal architecture. RPL24 localizes predominantly to the cytoplasm and nucleolus, reflecting its dual role in ribosome biogenesis and cytoplasmic translation.
The RPL24 antibody is widely used in molecular biology, cancer research, and translational control studies to analyze ribosome composition, protein synthesis rates, and growth regulation. Western blot analysis identifies an 18 kilodalton band corresponding to RPL24, while immunofluorescence shows strong cytoplasmic and nucleolar localization. This antibody supports research examining how ribosomal proteins coordinate mRNA translation with cellular metabolism and proliferation.
Functionally, RPL24 promotes ribosome maturation by assisting in 28S rRNA folding and subunit joining. Reduced RPL24 expression impairs translation efficiency and causes growth defects, while overexpression supports rapid proliferation in tumor cells. Mutations in ribosomal proteins including RPL24 are associated with developmental syndromes and cancers characterized by deregulated protein synthesis. The RPL24 antibody provides a critical tool for studying ribosome biogenesis, translational regulation, and ribosomal stress responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
