| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Ribophorin I was used as the immunogen for the RPN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPN1 Antibody / Ribophorin I is a anti-RPN1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32R32 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: RPN1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32R32, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, FACS
Biological background
Protein glycosylation is a critical post translational modification that ensures proper folding, stability, and function of many secreted and membrane proteins. Research using RPN1 antibody has shown that Ribophorin I contributes to substrate recognition and complex stability within the oligosaccharyltransferase machinery. By supporting efficient glycosylation, RPN1 plays a vital role in quality control and trafficking of glycoproteins through the secretory pathway.
Studies with RPN1 antibody have revealed that disruption of Ribophorin I function impairs N linked glycosylation, leading to misfolded proteins and activation of endoplasmic reticulum stress responses. Persistent dysfunction contributes to protein misfolding diseases and disorders of glycoprotein biosynthesis. This makes RPN1 a critical factor in maintaining protein homeostasis.
In cancer biology, RPN1 expression has been linked to tumor progression and therapeutic resistance. Research using RPN1 antibody has demonstrated that overexpression of Ribophorin I supports increased protein synthesis and folding capacity in tumor cells, enabling rapid proliferation and adaptation to stress. Elevated levels of RPN1 have been associated with poor prognosis in certain cancers, positioning it as a potential biomarker.
RPN1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein expression and size, immunohistochemistry localizes RPN1 in rough endoplasmic reticulum rich tissues, and immunofluorescence reveals perinuclear reticular distribution. These applications make RPN1 antibody indispensable in research on protein folding and secretion.
By providing validated RPN1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
