| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RPS19 recombinant protein (Position: M1-H145) was used as the immunogen for the RPS19 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPS19 Antibody / 40S ribosomal protein S19 is a anti-RPS19 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RPS19
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
40S ribosomal protein S19 is synthesized in the cytoplasm and imported into the nucleolus, where ribosome assembly begins. It binds pre-rRNA intermediates and contributes to proper folding and cleavage of the 18S rRNA precursor. The RPS19 antibody enables detection of this essential component within ribosomal particles, supporting studies of translational regulation and nucleolar dynamics. Its nucleolar localization reflects its participation in early 40S subunit maturation steps.
Mutations in RPS19 are the most common cause of Diamond-Blackfan anemia (DBA), a congenital erythroid aplasia characterized by defective red blood cell production. Loss of 40S ribosomal protein S19 impairs ribosome biogenesis, activates p53-mediated stress responses, and reduces erythroid progenitor survival. The RPS19 antibody supports investigation of these pathogenic mechanisms by enabling quantification of protein levels in patient-derived cells and experimental models. It also helps identify how ribosomal stress contributes to bone marrow failure and developmental abnormalities.
In addition to its ribosomal role, 40S ribosomal protein S19 has extra-ribosomal functions in apoptosis regulation, inflammation, and cell signaling. It can be released extracellularly where it acts as a chemotactic factor for macrophages. The RPS19 antibody provides a valuable means of analyzing these non-canonical functions, revealing how ribosomal proteins can serve signaling roles beyond translation. Dysregulation of RPS19 has also been observed in several cancers, linking ribosome synthesis with oncogenic growth control.
The RPS19 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry, yielding characteristic nucleolar and cytoplasmic staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
