| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RPS19BP1 recombinant protein (Position: M1-S136) was used as the immunogen for the RPS19BP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPS19BP1 Antibody / Ribosomal protein S19-binding protein 1 is a anti-RPS19BP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.
Key elements and design rationale
- Target: RPS19BP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
RPS19BP1 is predominantly localized to the nucleolus and nucleoplasm, where it contributes to the assembly of ribosomal subunits and maintenance of rRNA transcription. Depletion of RPS19BP1 results in reduced 40S ribosomal subunit formation and impaired protein synthesis. Given the central role of ribosome biogenesis in cell growth, dysregulation of RPS19BP1 affects proliferation and stress response pathways. The protein has been linked to hematopoietic development, as mutations or altered expression may influence erythropoiesis through its association with RPS19, a protein mutated in Diamond-Blackfan anemia.
The RPS19BP1 antibody is used in molecular biology and hematology research to study nucleolar function, ribosomal assembly, and cell growth control. Western blot analysis identifies a 58 kilodalton band corresponding to RPS19BP1, while immunofluorescence demonstrates strong nucleolar staining patterns. In proliferating cells, RPS19BP1 colocalizes with fibrillarin and other rRNA processing factors. Because nucleolar integrity is sensitive to stress, this antibody is also applied in experiments assessing nucleolar stress and its effects on p53 stabilization.
Beyond ribosome biogenesis, RPS19BP1 may have additional regulatory roles in chromatin remodeling and transcriptional control. Its interaction network includes several chromatin-binding and RNA-processing proteins. Aberrant expression of RPS19BP1 has been noted in certain cancers, linking ribosomal stress to tumorigenesis. The RPS19BP1 antibody therefore provides an essential tool for investigating nucleolar function, ribosomal disorders, and the interface between protein synthesis and cell cycle control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
