| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Rsk 2/MAPKAP Kinase 1b/RPS6KA3 recombinant protein (Position: D393-K733) was used as the immunogen for the RPS6KA3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPS6KA3 Antibody / Ribosomal protein S6 kinase alpha-3 is a anti-RPS6KA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: RPS6KA3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, IP, FACS, ELISA
Biological background
RPS6KA3 is encoded by the RPS6KA3 gene located on the X chromosome (Xp22.12). The protein is approximately 83 kilodaltons and contains two distinct kinase domains: an N-terminal kinase domain responsible for substrate phosphorylation and a C-terminal kinase domain that mediates activation through phosphorylation by ERK1/2. This dual-kinase configuration enables fine-tuned regulation of RPS6KA3 activity in response to mitogenic and stress stimuli.
The RPS6KA3 antibody detects the full-length kinase as well as phosphorylated intermediates in western blot and immunofluorescence assays. RPS6KA3 phosphorylates transcription factors such as CREB, c-Fos, and histone H3, linking MAPK activation to transcriptional control. It also phosphorylates cytoplasmic substrates involved in mRNA translation and cytoskeletal rearrangement. The protein plays a central role in synaptic plasticity and long-term memory formation, highlighting its importance in neural signaling.
Mutations in RPS6KA3 cause Coffin-Lowry syndrome, an X-linked developmental disorder characterized by cognitive impairment and skeletal abnormalities. Disruption of RPS6KA3 signaling leads to defective ERK pathway regulation, impaired neuronal differentiation, and altered gene expression. Overactivation of RPS6KA3, on the other hand, contributes to oncogenic transformation through hyperactive MAPK signaling, promoting cell proliferation and resistance to apoptosis.
Because of its role in growth and survival pathways, RPS6KA3 serves as a potential therapeutic target in cancer and neurological disease. The RPS6KA3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
