| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human RPS8 was used as the immunogen for the RPS8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RPS8 Antibody / Ribosomal protein S8 is a anti-RPS8 Rabbit antibody Recombinant Rabbit Monoclonal clone 30R72 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RPS8
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30R72, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, IP, FACS
Biological background
RPS8 antibody is widely applied in molecular biology, cancer research, and developmental biology. Ribosomal proteins serve not only structural roles but also regulate gene expression, cell growth, and stress responses. RPS8 has been implicated in ribosomopathies, tumorigenesis, and translational control. By detecting RPS8, researchers can study both canonical ribosome function and non ribosomal roles in cell regulation.
Applications for RPS8 antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects RPS8 protein in cell lysates, immunohistochemistry maps expression in tissues, and immunofluorescence reveals nucleolar and cytoplasmic localization. These techniques provide robust approaches for studying ribosome biogenesis and translation.
Altered ribosomal protein expression contributes to cancer, where increased protein synthesis supports uncontrolled proliferation. RPS8 overexpression has been observed in colorectal and lung cancers, where it correlates with tumor progression. Ribosomal stress caused by RPS8 deficiency activates p53 pathways, linking ribosome integrity to genome stability. By applying RPS8 antibody, scientists can explore how ribosomal proteins contribute to disease biology.
Beyond cancer, RPS8 is studied in development and neurobiology. Ribosome assembly defects cause congenital disorders characterized by growth delay, anemia, and neurological symptoms. RPS8 participates in translational regulation during neuronal differentiation and synaptic activity. Detection with antibody based assays supports research into ribosomopathies and neurodevelopmental syndromes.
RPS8 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
