| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RSL24D1 recombinant protein (Position: M1-P163) was used as the immunogen for the RSL24D1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RSL24D1 Antibody / Ribosomal L24 domain-containing protein 1 is a anti-RSL24D1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleolus.
Key elements and design rationale
- Target: RSL24D1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, RSL24D1 antibody identifies a 332-amino-acid nucleolar protein that associates with preribosomal complexes during 60S subunit assembly. RSL24D1 interacts with rRNA intermediates and ribosomal proteins to facilitate cleavage and folding events required for ribosome maturation. It participates in the late steps of rRNA processing, ensuring proper incorporation of 28S rRNA into the large subunit. RSL24D1 is functionally conserved across eukaryotes, underscoring its central role in ribosome biogenesis.
The RSL24D1 gene is located on chromosome 15q25.2 and is expressed in proliferative tissues such as bone marrow, testis, and liver, where active ribosome production supports rapid cell growth. Expression levels correlate with translational demand and are tightly controlled by growth factor and nutrient signaling pathways that regulate ribosome assembly.
Pathologically, dysregulation of RSL24D1 affects ribosomal homeostasis and has been linked to cancer and ribosomopathies. Overexpression promotes cell proliferation and is associated with tumor growth, while loss of function leads to impaired ribosome production and nucleolar stress. RSL24D1 interacts with tumor suppressor pathways, including p53 activation in response to ribosomal imbalance. Research using RSL24D1 antibody supports studies in translation regulation, cancer biology, and ribosome assembly mechanisms.
RSL24D1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar ribosome assembly proteins.
Structurally, Ribosomal L24 domain-containing protein 1 contains a conserved L24-like domain characteristic of large ribosomal subunit proteins, facilitating interactions with rRNA and assembly factors. Its C-terminal region mediates nucleolar localization and binding to preribosomal complexes. This antibody enables detailed investigation of RSL24D1's role in ribosome biogenesis, translational control, and cellular growth regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
