| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RSPO4 recombinant protein (Position: R60-K206) was used as the immunogen for the RSPO4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RSPO4 Antibody / R-spondin-4 is a anti-RSPO4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: RSPO4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
R-spondin-4 is characterized by two cysteine-rich furin-like domains that mediate receptor binding and a thrombospondin type 1 (TSP1) domain that influences protein localization. Through these domains, R-spondin-4 stabilizes Frizzled and LRP5/6 receptors on the cell surface, prolonging Wnt pathway activation. The RSPO4 antibody helps researchers examine this process by detecting protein expression in tissues where Wnt signaling drives growth and differentiation, including skin, limb, and nail matrix.
Mutations in RSPO4 cause anonychia congenita, a rare developmental disorder marked by complete or partial absence of nails. Such mutations impair Wnt pathway activation, underscoring R-spondin-4's essential role in ectodermal appendage development. The RSPO4 antibody is invaluable for exploring these mechanisms, allowing measurement of protein levels and localization in affected tissues. In experimental systems, recombinant R-spondin-4 is used to stimulate stem cell proliferation and differentiation, emphasizing its relevance in tissue engineering and regenerative therapies.
Beyond development, R-spondin-4 has been implicated in tumorigenesis, particularly in cancers exhibiting aberrant Wnt signaling such as colorectal and gastric cancer. The RSPO4 antibody allows assessment of expression patterns in tumor samples and helps determine how altered RSPO4 activity contributes to cancer cell proliferation. In stem cell biology, this protein functions as a niche signal that maintains progenitor cell populations, making it a target of interest in organoid culture and tissue regeneration research.
The RSPO4 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields strong staining of secreted and extracellular matrix-associated R-spondin-4.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
