| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RSRC2 recombinant protein (Position: F226-V434) was used as the immunogen for the RSRC2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RSRC2 Antibody / Arginine/serine-rich coiled-coil protein 2 is a anti-RSRC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: RSRC2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Functionally, RSRC2 antibody identifies a 477-amino-acid protein localized to nuclear speckles where it associates with splicing machinery components. RSRC2 facilitates exon recognition and splice site selection through interactions with U2AF and SR proteins, ensuring accurate pre-mRNA splicing. It also acts as a transcriptional co-regulator linking splicing to gene expression control.
The RSRC2 gene is located on chromosome 12q24.31 and is widely expressed, with enrichment in brain, heart, and testis. RSRC2 activity supports tissue-specific splicing patterns essential for cellular differentiation and development. It dynamically redistributes within the nucleus in response to transcriptional and stress signals, reflecting its role in gene expression regulation.
Pathologically, RSRC2 dysregulation has been implicated in neurodegenerative disease, cancer, and cardiovascular disorders. Altered RSRC2 expression disrupts mRNA splicing fidelity and may affect tumor suppressor or oncogene expression. Research using RSRC2 antibody aids in studies of RNA processing, splicing regulation, and gene expression control.
RSRC2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect splicing regulators and nuclear speckle proteins.
Structurally, Arginine/serine-rich coiled-coil protein 2 contains SR repeats mediating protein interactions and a coiled-coil region that promotes nuclear localization and complex formation. This antibody enables detailed analysis of RSRC2's role in co-transcriptional mRNA processing and cellular homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
