| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human C14orf166/RTRAF recombinant protein (Position: M1-R244) was used as the immunogen for the RTRAF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
RTRAF Antibody / hCLE is a anti-RTRAF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: RTRAF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, ELISA
Biological background
RTRAF is encoded by the RTRAF gene located on human chromosome 17q23.3. The protein is approximately 375 amino acids long and localizes to both the nucleus and cytoplasm, reflecting its diverse functions. It forms complexes with RNA polymerase II, transcription elongation factors, and RNA helicases, linking transcription with RNA processing and export. RTRAF also associates with translation initiation factor eIF3, regulating mRNA translation efficiency.
The RTRAF antibody detects a 43 kilodalton band by western blot and demonstrates both nuclear speckle and cytoplasmic punctate staining in immunofluorescence assays. RTRAF participates in gene expression control by influencing promoter clearance, mRNA splicing, and ribosome loading. Its loss disrupts RNA metabolism, leading to reduced protein synthesis and altered stress granule dynamics.
Functionally, RTRAF has been linked to viral replication, as it enhances viral RNA translation and replication complex assembly in infected cells. In cancer, RTRAF expression correlates with increased proliferation and tumor aggressiveness due to its role in sustaining translation of growth-related mRNAs. It also contributes to chromatin organization by interacting with histone modifiers and transcriptional regulators.
Through its integration of transcription, RNA transport, and translation, RTRAF acts as a key coordinator of gene expression homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
