| Field | Specification |
|---|---|
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
RUNX2 (runt-related transcription factor 2) is a master transcription factor for osteoblast differentiation and skeletal development, essential for both intramembranous and endochondral ossification. By activating bone matrix genes such as osteocalcin, RUNX2 drives osteoblast maturation and mineralization. Loss-of-function mutations cause cleidocranial dysplasia, a disorder of delayed skeletal ossification, while aberrant RUNX2 activity has been implicated in pathological fibroblast conversion during lung fibrosis, including idiopathic pulmonary fibrosis. RUNX2 activity can be detected through composite elements that combine an FGF-responsive element and the RUNX2-responsive OSE2 element, capturing transcriptional synergy between FGF signaling and active RUNX2.
Product Description & Applications
The RUNX2 Reporter Lentivirus is a transcription-factor reporter system for detecting RUNX2-mediated transcriptional activity. It contains tandem repeats of a composite regulatory element combining the osteocalcin FGF response element (OCFRE) and the RUNX2-responsive OSE2 element from the rat osteocalcin promoter, enabling detection of synergy between FGF-induced signaling and RUNX2 activation; reporter activation requires active RUNX2. The elements are coupled to a minimal TATA-box promoter and an upstream enhancer that maximizes signal-to-noise to drive a fluorescent or luminescent reporter (including BFP2, d2GFP, EGFP, GFP, mCherry, RFP, firefly, Gaussia, or Renilla luciferase), with a constitutively expressed selection marker for stable polyclonal cell line generation. It is used to study RUNX2 activity in osteoblast biology, bone development, and fibrosis research. Supplied as purified lentiviral particles.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RUNX2 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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