Ryanodine

Product details

• Chemical name: 1H-Pyrrole-2-carboxylic acid,(3S,4R,4aR,6S,7S,8R,8aS,8bR,9S,9aS)-dodecahydro-4,6,7,8 a,8b,9a-hexahydroxy-3,6a,9-trimethyl-7-(1-methylethyl)- 6,9-methanobenzo[1,2]pentaleno[1,6-bc]furan-8-yl ester.
• CAS number: 15662-33-6
• Molecular formula: C25H35NO9.
• Purity: >98%
• Concentration: 1-25 μM.
• Form: Lyophilized Powder
• Solubility: Methanol. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: Ryanodine receptors
• Source: Natural
• Activity: Ryanodine is a blocker of the Ryanodine receptor (RyR) Ca2+ release channel1.

Scientific background

Ryanodine is found naturally in the stem and roots of the plant Ryania speciosa. The ryanodine alkaloid was first isolated as a potential insecticide1 but was found to act as a potent paralytic on skeletal and cardiac muscles.2 Ryanodine inhibits SR Ca2+ release by binding with high affinity to the ryanodine receptor (RyR) channel as a tetrameric complex.3,4The RyR channel has three isoforms and is a poorly selective Ca2+ channel (selectivity Ca2+/K+ ~6) with very high conductance (~700 pS with K+ as charge carrier and ~100 pS with Ca2+ as charge carrier). The channel is regulated by Ca2+, Mg2+, ATP, and caffeine. Application of ryanodine locks the channel in a slow-gating subconductance state. Opening of the ryanodine-bound channel is long-lived and gives rise to a smaller unit current (~1/3 or ~1/2) than the control amplitude. This action of ryanodine on single RyR channel function is quite distinctive and is often used for functional identification of the channel.5-8Ryanodine binds with high affinity to RyR proteins in a pore located in the carboxyl terminus of the protein.9 Binding induces a complex change in single RyR channel function. This change is similar in all three RyR channel isoforms, inducing open events of very long duration and simultaneous reduction of ion conductance through the pore.10,7 This dual impact on single-channel function (i.e. slower gating and reduced conductance) suggests a complicated mode of action. This is also supported by the rather complicated ryanodine dose dependency. Low doses of ryanodine (~10 nM) are reported to increase the frequency of single RyR channel openings to the normal conductance level. Intermediate ryanodine doses (~1 µM) are reported to induce the classical ryanodine action described above. High doses of ryanodine (~100 µM) are reported to lock the channel in a closed configuration.11,10It is generally assumed that each RyR monomer contains one high-affinity ryanodine binding site (KD

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

Cited application(s)

Electrophysiology

• SDS (PDF)

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