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| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human S100P was used as the immunogen for the S100P antibody. |
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Overview
S100P Antibody / Protein S100-P is a anti-S100P Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: S100P
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS
Biological background
S100P is expressed in multiple tissues, including placenta, pancreas, lung, and prostate, and is frequently upregulated in cancers where it contributes to tumor progression and metastasis. The protein interacts with the receptor for advanced glycation end products (RAGE) at the cell surface, activating downstream MAPK and NF-kB pathways that promote proliferation, migration, and resistance to apoptosis. Elevated S100P expression has been associated with poor prognosis in breast, pancreatic, and colorectal carcinomas, making it a biomarker for aggressive tumor phenotypes and potential therapeutic target.
The S100P antibody is widely used in cancer biology and molecular pathology to study calcium signaling and metastasis regulation. In western blot analysis, S100P appears as an approximately 11 kilodalton band corresponding to the monomeric form, while immunohistochemistry reveals both cytoplasmic and nuclear localization depending on cell type. In cancer diagnostics, S100P immunoreactivity is employed to distinguish pancreatic ductal adenocarcinoma from benign pancreatic lesions and to evaluate metastatic spread. The antibody is also valuable in studies of placental function and epithelial differentiation where S100P participates in hormone-responsive signaling networks.
Beyond oncology, S100P contributes to inflammation and tissue repair. It enhances fibroblast motility and collagen remodeling through calcium-dependent activation of small GTPases such as Rac1 and Cdc42. It also regulates gene transcription by interacting with nuclear receptors and coactivators, influencing expression of stress-response and survival genes. The S100P antibody enables detailed characterization of these pathways, providing insight into the molecular mechanisms that couple calcium signaling to cell behavior. Researchers employ it in western blotting, immunofluorescence, and immunohistochemistry to quantify expression and localization in tissue or cultured cells.
Because of its strong link to metastasis, S100P has emerged as a therapeutic target and diagnostic biomarker. Inhibitors of the S100P-RAGE interaction are under investigation for their potential to reduce tumor invasion and inflammation. The S100P antibody supplied by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
