| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SAFB was used as the immunogen for the SAFB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SAFB Antibody / Scaffold attachment factor B is a anti-SAFB Rabbit antibody Recombinant Rabbit Monoclonal clone 30S09 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SAFB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30S09, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
SAFB antibody is widely applied in molecular biology, transcriptional regulation, and cancer research. SAFB associates with nuclear matrix elements and modulates expression of hormone responsive genes. It also functions as a corepressor of estrogen receptor alpha, impacting signaling pathways critical in breast cancer biology. By detecting SAFB, researchers can explore mechanisms of gene regulation at the interface of DNA, RNA, and protein interactions.
Western blot assays detect SAFB protein in nuclear extracts, while immunohistochemistry maps expression in hormone responsive tissues and tumors. Immunofluorescence highlights nuclear speckle localization, consistent with its role in transcriptional complexes. These applications enable detailed characterization of SAFB across tissues and conditions.
SAFB also contributes to stress response pathways. It regulates heat shock protein gene expression and participates in apoptosis and cell cycle control. Mutations or altered expression of SAFB have been linked to breast cancer, prostate cancer, and neurological disorders. By applying SAFB antibody, scientists can evaluate its diverse functions in gene regulation, hormone signaling, and disease pathogenesis.
SAFB antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
