| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SALL2 recombinant protein (Position: E23-H972) was used as the immunogen for the SALL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SALL2 Antibody / Spalt-like transcription factor 2 is a anti-SALL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: SALL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
SALL2 is encoded by the SALL2 gene located on human chromosome 14q11.2. The protein localizes primarily to the nucleus and regulates genes controlling cell proliferation, apoptosis, and differentiation. During development, SALL2 contributes to optic nerve formation, limb morphogenesis, and neural tube closure. It interacts with other transcription factors such as p53, WT1, and SP1, integrating growth factor signaling with chromatin remodeling. Its expression is regulated by developmental cues and stress responses, suggesting broad involvement in maintaining genomic stability and tissue integrity.
The SALL2 antibody detects multiple isoforms ranging from 100-150 kDa depending on splicing and post-translational modifications. Western blot analyses demonstrate nuclear enrichment, while immunohistochemistry reveals SALL2 expression in neural tissues, retina, and developing mesenchyme. Functionally, SALL2 acts as a tumor suppressor by promoting p21 expression and inhibiting uncontrolled cell proliferation. Loss or downregulation of SALL2 has been associated with glioblastoma, ovarian carcinoma, and other cancers. Conversely, aberrant expression can disrupt differentiation programs and promote oncogenic transformation under certain signaling conditions.
Research has also revealed SALL2's role in stem cell pluripotency and differentiation. It interacts with key developmental regulators, including OCT4 and SOX2, and influences chromatin accessibility at developmental gene loci. In neural systems, SALL2 supports axon guidance and neuronal survival, linking it to neurological disorders when dysregulated.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
