| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SASS6 recombinant protein (Position: E101-S657) was used as the immunogen for the SASS6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SASS6 Antibody / Spindle assembly abnormal protein 6 is a anti-SASS6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Centrosome.
Key elements and design rationale
- Target: SASS6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, IHC, WB, FACS
Biological background
Loss of SASS6 disrupts centriole formation, resulting in monopolar spindles and abnormal cell division. Mutations in SASS6 cause primary microcephaly, characterized by reduced brain size and defective neural progenitor proliferation. The SASS6 antibody allows detection of centriolar structures in cultured cells and tissue samples. Immunofluorescence using this antibody reveals punctate staining at centrosomes during interphase and mitosis, while western blotting identifies a 65 kilodalton band. Because of its critical role in centrosome biogenesis, SASS6 serves as a core marker for centriole assembly studies and cell cycle regulation research.
Beyond its role in structural assembly, SASS6 interacts with STIL and CEP135 to coordinate recruitment of microtubule-nucleating proteins. Its regulated degradation through the APC/C complex ensures centriole duplication occurs only once per cell cycle. Overexpression of SASS6 leads to centrosome amplification, a phenotype frequently observed in cancer cells. The SASS6 antibody is thus valuable for examining centrosome abnormalities and chromosomal instability associated with tumorigenesis. Researchers also employ this antibody in developmental studies to trace centriole formation during early embryogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
