| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SBK1 recombinant protein (Position: E34-Q293) was used as the immunogen for the SBK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SBK1 Antibody / SH3 domain-binding kinase 1 is a anti-SBK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SBK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IP, FACS, ELISA
Biological background
Functionally, SBK1 antibody identifies a 364-amino-acid cytoplasmic and membrane-associated kinase that contains an N-terminal kinase domain and an SH3-binding motif. SBK1 is expressed primarily in the brain, where it interacts with SH3 domain-containing proteins involved in signal transduction and vesicular trafficking. Although its substrates remain incompletely characterized, SBK1 likely modulates signaling pathways linked to neuronal differentiation and synaptic strength.
The SBK1 gene is located on chromosome 16q12.1 and is expressed in brain, skeletal muscle, and heart. Expression levels are regulated by neuronal activity and stress stimuli, suggesting involvement in activity-dependent signaling cascades. SBK1 may function as part of kinase networks that fine-tune cellular responses to excitatory signals and metabolic stress.
Pathologically, altered SBK1 expression has been observed in neurological disorders and cancers. Increased expression has been associated with gliomas and neural tissue remodeling, while reduced expression may impair neuronal communication or contribute to neurodegenerative processes. Research using SBK1 antibody supports studies in neuroscience, kinase signaling, and stress adaptation pathways.
SBK1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect neuronal kinases.
Structurally, SH3 domain-binding kinase 1 contains a conserved catalytic domain with ATP-binding and substrate recognition motifs characteristic of serine/threonine kinases. Its SH3-binding region facilitates association with signaling scaffolds and adaptor proteins. This antibody enables exploration of SBK1�s role in neuronal signaling, cytoskeletal regulation, and adaptive stress responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
