| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SFRS2IP/SCAF11 recombinant protein (Position: D81-D1399) was used as the immunogen for the SCAF11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCAF11 Antibody / SR-related CTD-associated factor 11 / SFRS2IP is a anti-SCAF11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: SCAF11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Functionally, SCAF11 antibody identifies a 1,450-amino-acid nuclear protein containing RNA recognition motifs (RRMs) and arginine/serine-rich (RS) domains characteristic of SR proteins. SCAF11 binds to the C-terminal domain (CTD) of RNA polymerase II, recognizing its phosphorylation state and linking elongating polymerases to RNA splicing machinery. It interacts with splicing factors such as SRSF2 and SF3B1 and participates in the coordination of cotranscriptional mRNA processing.
The SCAF11 gene is located on chromosome 12q24.33 and is ubiquitously expressed, with higher levels in actively proliferating and transcriptionally active cells. Expression peaks during S-phase and in tissues with elevated RNA synthesis such as liver and brain. SCAF11 plays a dual role as a structural component of the transcriptional complex and a modulator of RNA maturation efficiency.
Pathologically, dysregulation of SCAF11 has been linked to aberrant RNA processing and oncogenic transformation. Overexpression contributes to cancer progression by promoting transcriptional elongation of growth-associated genes, while knockdown disrupts RNA stability and gene expression homeostasis. SCAF11 has also been implicated in neurodegenerative conditions where transcriptional stress affects mRNA splicing fidelity. Research using SCAF11 antibody supports studies in transcriptional regulation, RNA processing, and chromatin biology.
SCAF11 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcription-associated RNA-binding proteins.
Structurally, SR-related CTD-associated factor 11 contains multiple RRMs for RNA binding and long RS-rich regions that mediate protein-protein interactions with splicing and elongation factors. Its CTD-binding domain recognizes phosphorylated Ser2 residues on RNA polymerase II, positioning SCAF11 at sites of active transcription. This antibody facilitates investigation of SCAF11's role in coordinating transcription, RNA processing, and post-transcriptional regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
