| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCAMP3 recombinant protein (Position: M1-P347) was used as the immunogen for the SCAMP3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCAMP3 Antibody / Secretory carrier-associated membrane protein 3 is a anti-SCAMP3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Vesicles).
Key elements and design rationale
- Target: SCAMP3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, IF, IHC, ICC, WB, FACS
Biological background
SCAMP3 is encoded by the SCAMP3 gene located on human chromosome 1q24.3. The protein is approximately 347 amino acids in length and contains four transmembrane domains along with cytoplasmic N- and C-terminal regions that interact with clathrin-associated sorting proteins and ubiquitin machinery. It localizes to early and late endosomes, Golgi apparatus, and the plasma membrane depending on cellular trafficking conditions.
The SCAMP3 antibody detects a 38 kilodalton protein by western blot and reveals punctate cytoplasmic staining corresponding to endosomal vesicles under confocal microscopy. SCAMP3 controls receptor fate by regulating cargo deubiquitination and sorting into intraluminal vesicles destined for lysosomal degradation. It acts downstream of the ESCRT (endosomal sorting complex required for transport) machinery and modulates the recycling of growth factor receptors such as EGFR and transferrin receptor.
Loss or knockdown of SCAMP3 disrupts receptor recycling, causing accumulation of ubiquitinated cargo and altered signaling duration. Overexpression can inhibit receptor downregulation and sustain proliferative signaling. Through its dual regulation of endocytosis and exocytosis, SCAMP3 maintains balance between degradation and recycling of membrane proteins, affecting cellular homeostasis and response to extracellular cues.
SCAMP3 also participates in cytokine secretion, exosome biogenesis, and viral budding, emphasizing its broad role in vesicle-mediated processes. Dysregulation has been associated with cancer progression, immune modulation, and neurodegenerative disorders where vesicle trafficking is impaired. Because SCAMP3 serves as a molecular switch between degradation and recycling, it provides insight into receptor signaling dynamics.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
