| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCAP recombinant protein (Position: F1224-D1279) was used as the immunogen for the SCAP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCAP Antibody / SREBP cleavage-activating protein is a anti-SCAP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SCAP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
SREBP cleavage-activating protein contains multiple transmembrane domains and a sterol-sensing domain that binds cholesterol. Under high-cholesterol conditions, SCAP associates with insulin-induced gene (INSIG) proteins in the ER, retaining SREBPs in an inactive state. When sterol levels fall, this interaction is disrupted, allowing SCAP–SREBP complexes to move to the Golgi. There, site-1 and site-2 proteases release the active SREBP transcription factor, which then migrates to the nucleus to induce lipid metabolism genes. The SCAP antibody enables researchers to track this dynamic process, quantifying SCAP expression and subcellular localization under various lipid conditions.
As a central regulator of lipid homeostasis, SREBP cleavage-activating protein plays critical roles in energy balance, membrane synthesis, and metabolic adaptation. Dysregulation of SCAP contributes to disorders such as hyperlipidemia, fatty liver disease, obesity, and atherosclerosis. The SCAP antibody supports investigation of these metabolic conditions by detecting protein expression in liver, adipose tissue, and other lipid-synthesizing organs. In cellular studies, it aids analysis of sterol-dependent trafficking and SREBP activation using immunoblotting and immunofluorescence techniques.
In addition to cholesterol regulation, SCAP participates in broader metabolic pathways influenced by insulin signaling and nutrient status. Altered SCAP activity has been linked to metabolic reprogramming in cancer cells, where enhanced lipid biosynthesis supports rapid proliferation. The SCAP antibody can be used to study these mechanisms and assess the impact of pharmacologic SCAP inhibition on lipid synthesis and growth control. Western blotting and immunohistochemistry using this antibody reveal SCAP's distribution across the ER and Golgi, providing a spatial understanding of its sterol-sensing function.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
