| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCARA5 recombinant protein (Position: K31-R288) was used as the immunogen for the SCARA5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCARA5 Antibody / Scavenger receptor class A member 5 is a anti-SCARA5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SCARA5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, SCARA5 antibody identifies a 495-amino-acid receptor consisting of a collagen-like domain, a coiled-coil stalk, and a C-terminal scavenger receptor cysteine-rich domain that binds various ligands. SCARA5 acts as a ferritin receptor, mediating non-transferrin-dependent iron uptake. It also recognizes bacterial components and oxidized lipoproteins, linking innate immune recognition with metabolic regulation.
The SCARA5 gene is located on chromosome 8p21.1 and is highly expressed in kidney, lung, and heart. SCARA5 localizes to the plasma membrane and endocytic vesicles, facilitating internalization of ferritin and other ligands. It contributes to maintaining iron homeostasis and protecting tissues from oxidative stress caused by excess free iron.
Pathologically, downregulation of SCARA5 expression has been reported in several cancers, including hepatocellular and renal carcinoma, suggesting a tumor suppressive role. By controlling iron uptake and reactive oxygen species balance, SCARA5 helps limit oxidative damage and cell proliferation. Research using SCARA5 antibody aids in studying iron metabolism, scavenger receptor function, and innate immunity.
SCARA5 antibody is validated for western blotting, immunohistochemistry, and flow cytometry to detect receptor expression in tissues and cells.
Structurally, Scavenger receptor class A member 5 features a collagenous triple-helical domain that promotes trimerization and ligand binding, resembling other scavenger receptor family members. This antibody enables analysis of SCARA5�s contribution to innate immunity, oxidative stress defense, and cancer suppression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
