| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCD recombinant protein (Position: R26-S358) was used as the immunogen for the SCD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCD1 Antibody / Stearoyl-CoA desaturase is a anti-SCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: ER.
Key elements and design rationale
- Target: SCD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IP, IF, ICC, WB
Biological background
SCD1 is encoded by the SCD gene located on human chromosome 10q24.31. The enzyme is approximately 41 kilodaltons and contains four transmembrane domains that anchor it to the ER membrane, where it introduces a cis-double bond between the ninth and tenth carbon atoms of saturated fatty acyl-CoAs. The desaturation reaction requires oxygen, NADH, and cytochrome b5, forming the core step in the synthesis of monounsaturated fatty acids.
The SCD1 antibody typically detects a 36-42 kilodalton band by western blot and reveals reticular ER staining in immunofluorescence microscopy. SCD1 plays a vital role in lipid homeostasis by controlling the ratio of saturated to unsaturated fatty acids, influencing membrane composition, lipoprotein secretion, and energy storage.
Upregulation of SCD1 promotes lipogenesis and is associated with obesity, insulin resistance, and hepatic steatosis. Conversely, inhibition or genetic deletion of SCD1 enhances fatty acid oxidation and protects against metabolic disease. SCD1 expression is regulated by sterol regulatory element-binding protein 1c (SREBP1c) and peroxisome proliferator-activated receptors (PPARs), linking it to dietary and hormonal control of lipid metabolism.
Beyond metabolism, SCD1 influences inflammation, cancer cell proliferation, and stem cell maintenance. High SCD1 expression correlates with poor prognosis in several cancers due to its role in sustaining membrane synthesis and redox balance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
