| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCLT1 recombinant protein (Position: F19-I688) was used as the immunogen for the SCLT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCLT1 Antibody / Sodium channel and clathrin linker 1 is a anti-SCLT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SCLT1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
SCLT1 is encoded by the SCLT1 gene located on human chromosome 4q28.1. The protein is approximately 68 kilodaltons and is conserved among vertebrates. It contains coiled-coil regions that mediate binding to other distal appendage proteins, including CEP164, FBF1, and CEP83. SCLT1 provides structural linkage between microtubule-based centrioles and the membrane docking machinery required for ciliogenesis initiation. Knockout or mutation of SCLT1 disrupts distal appendage integrity and prevents cilia formation.
The SCLT1 antibody detects the protein by western blot as a 68 kilodalton band and reveals punctate centrosomal staining under immunofluorescence microscopy. Localization studies show that SCLT1 is present at the distal end of the mother centriole and co-localizes with ciliary initiation markers. Functional analyses demonstrate that SCLT1 depletion inhibits ciliary vesicle docking, leading to defective Hedgehog signaling and altered tissue morphogenesis. Mutations in SCLT1 have been linked to ciliopathies such as Senior-Loken syndrome and orofaciodigital spectrum disorders, highlighting its importance in organ development.
Beyond its role in ciliogenesis, SCLT1 may interact with ion channels and clathrin adaptor proteins, potentially linking ciliary membrane organization with endocytic trafficking. It has also been implicated in cell cycle control through centrosome duplication regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
