| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SCO2 recombinant protein (Position: Q29-S266) was used as the immunogen for the SCO2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SCO2 Antibody / Copper chaperone SCO2 is a anti-SCO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: SCO2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Located on chromosome 22q13.33, SCO2 (Synthesis of Cytochrome c Oxidase 2) is one of two homologous human SCO genes, the other being SCO1. Both are nuclear-encoded but localized to mitochondria, where they coordinate copper homeostasis. SCO2 binds copper ions via conserved cysteine and histidine residues and transfers them to cytochrome oxidase subunits through direct protein-protein interactions. Mutations in SCO2 disrupt this process and result in cytochrome c oxidase deficiency, leading to diseases such as fatal infantile cardioencephalomyopathy and Leigh-like syndrome.
SCO2 expression is regulated by hypoxia and cellular copper status. Under low oxygen conditions, transcription factor HIF-1 may influence SCO2 expression to modulate oxidative phosphorylation efficiency. The protein is also involved in maintaining redox balance and preventing the accumulation of reactive oxygen species (ROS) within mitochondria. Beyond its role in energy production, SCO2 contributes to cellular copper distribution and may participate in signaling pathways that coordinate mitochondrial biogenesis.
Immunohistochemical analysis using SCO2 antibody demonstrates mitochondrial localization in heart, skeletal muscle, and brain tissues, consistent with its role in energy metabolism. The antibody serves as a key tool for studying mitochondrial function, copper metabolism, and respiratory chain biogenesis. SCO2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
