| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human SDHC was used as the immunogen for the SDHC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SDHC Antibody / Succinate dehydrogenase complex subunit C is a anti-SDHC Rabbit antibody Recombinant Rabbit Monoclonal clone 32S26 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SDHC
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32S26, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
SDHC antibody is widely used in studies of mitochondrial biology, cancer research, and metabolic disorders. Mutations in the SDHC gene are associated with hereditary paragangliomas and pheochromocytomas, tumors that arise from defects in mitochondrial function. Detecting SDHC expression with specific antibodies allows researchers to explore the molecular basis of these diseases and evaluate potential diagnostic markers.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, SDHC antibody specifically recognizes the SDHC protein band, providing accurate analysis of expression levels. In immunohistochemistry, it highlights mitochondrial localization patterns within tissue sections, while immunofluorescence reveals subcellular distribution in cultured cells. These applications make SDHC antibody versatile for both basic and translational research.
SDHC plays an essential role in linking the TCA cycle to oxidative phosphorylation. Disruption of SDHC function impairs both energy production and reactive oxygen species regulation, contributing to tumorigenesis and metabolic disease. Studies employing SDHC antibody provide insights into how alterations in complex II activity influence cancer metabolism, oxidative stress, and cell survival.
Beyond oncology, SDHC antibody is valuable for investigating mitochondrial disorders, neurodegeneration, and cardiovascular diseases. Complex II dysfunction contributes to pathologies ranging from Leigh syndrome to ischemia-reperfusion injury, making SDHC an important focus of mitochondrial research. By detecting SDHC expression, researchers can evaluate mitochondrial integrity and assess therapeutic strategies aimed at restoring respiratory function.
SDHC antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
