| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human SECISBP2 recombinant protein (Position: E12-L854) was used as the immunogen for the SECISBP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SECISBP2 Antibody / Selenocysteine insertion sequence-binding protein 2 is a anti-SECISBP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nucleus, Miochondria.
Key elements and design rationale
- Target: SECISBP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Structurally, SECISBP2 contains RNA recognition motifs and a lysine-rich domain essential for binding SECIS elements. It forms part of a ribonucleoprotein complex with eukaryotic elongation factor EEFSEC and selenocysteine-specific tRNA (tRNA-Sec), ensuring accurate decoding of UGA as selenocysteine instead of a stop signal. SECISBP2 belongs to the SECIS-binding protein family, which includes SECISBP2L, a paralog with partially overlapping function. Co-localization studies show SECISBP2 distributed in cytoplasmic foci associated with ribosomes and endoplasmic reticulum-bound polysomes, indicating its role in co-translational selenoprotein synthesis.
Functionally, SECISBP2 regulates the expression of numerous selenoproteins involved in antioxidant defense, redox regulation, and thyroid hormone metabolism. These include glutathione peroxidases, thioredoxin reductases, and deiodinases. By controlling selenocysteine incorporation efficiency, SECISBP2 influences cellular responses to oxidative stress and selenium availability. During development, SECISBP2 expression supports organogenesis and metabolic maturation by maintaining proper selenoprotein production.
Mutations in SECISBP2 cause a multisystem disorder known as selenoprotein deficiency syndrome, characterized by impaired thyroid hormone activation, low plasma selenium levels, and muscle weakness. Reduced SECISBP2 function decreases synthesis of key antioxidant enzymes, leading to increased oxidative damage. Conversely, overexpression can enhance cellular resistance to oxidative stress by upregulating selenoprotein synthesis. Pathway associations include selenoamino acid metabolism, translational control, and oxidative stress response. Known binding partners include EEFSEC, SEPSECS, and tRNA-SEC, forming the machinery required for selenocysteine translation.
The SECISBP2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
