SELL Antibody / L-Selectin / LAM-1

Overview

SELL Antibody / L-Selectin / LAM-1 is an antibody targeting SELL, raised in Rabbit for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: SELL.
• Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Purified.
• Species reactivity: Human.
• Listed applications: WB, IHC-P, FACS (refer to on-page specifications for application-specific guidance).

Biological background

L-Selectin, also known as CD62L, SELL and LAM-1, is a cell adhesion molecule found on the surface of leukocytes, or white blood cells. It plays a role in the immune response by facilitating the binding of leukocytes to endothelial cells in the blood vessels, allowing them to migrate to sites of infection or inflammation. One of the key functions of L-Selectin is its involvement in the process of leukocyte rolling. When an infection or injury occurs, inflammatory signals are released that trigger the expression of adhesion molecules on the surface of endothelial cells. L-Selectin on leukocytes then binds to its ligands on the endothelial cells, causing the leukocytes to roll along the vessel wall. This rolling motion slows down the leukocytes and allows them to survey the endothelial surface for signs of infection. Once the leukocytes have identified the site of infection, they can firmly adhere to the endothelial cells and extravasate, or migrate, into the surrounding tissue. L-Selectin plays a critical role in this step by mediating the initial interaction between leukocytes and endothelial cells. Without L-Selectin, leukocytes would not be able to efficiently migrate to the site of infection, impairing the immune response.
In addition to its role in leukocyte trafficking, L-Selectin also plays a role in regulating the activation and function of leukocytes. Studies have shown that L-Selectin signaling can modulate the production of cytokines and chemokines, which are key mediators of inflammation. By influencing the inflammatory response, L-Selectin can help regulate the magnitude and duration of immune reactions, preventing excessive tissue damage. Furthermore, L-Selectin has been implicated in a variety of inflammatory and autoimmune diseases. Dysregulation of L-Selectin expression or function can lead to abnormal leukocyte trafficking and contribute to chronic inflammation. Understanding the role of L-Selectin in these conditions may provide insights into potential therapeutic targets for treating inflammatory diseases.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.