| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Immunogen | Recombinant Protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| UniProt # |
Product Overview
Perforin, a pore-forming protein found in the granules of cytotoxic T-lymphocytes and natural killer cells, functions to enable granzymes to enter the target cells and activate apoptosis. It has been suggested that perforin plays a role in alloimmunity, being involved in both the cytolytic process of rejection as well as downregulation of the T-cell mediated responses associated with the alloimmune response. Perforin-mediated cytotoxicity has also been linked to a number of autoimmune diseases.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| ELISA | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
Product Specifications
| Clone | IHC646 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG2 |
| Species Reactivity | Human |
| Storage | This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. |
Immunogen / Antigen
Recombinant Protein
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Citation Request
If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. Please include the manufacturer catalog number in your methods section to support reproducibility.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for ELISA, IHC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Recombinant Protein
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
