| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Septin 8 was used as the immunogen for the SEPTIN8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
SEPTIN8 Antibody / Septin 8 is a anti-SEPTIN8 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S14 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: SEPTIN8
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30S14, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
Septins form hetero oligomeric complexes that polymerize into filaments and higher order structures. Research using SEPTIN8 antibody has shown that Septin 8 contributes to filament assembly, acting as a structural scaffold at the plasma membrane and division sites. Septin complexes coordinate with actin and microtubules to regulate cell polarity and compartmentalization, ensuring proper cell division and morphogenesis.
In neurons, Septin 8 plays roles in dendritic branching and synapse formation. Studies with SEPTIN8 antibody have demonstrated that it localizes to dendritic spines and regulates cytoskeletal rearrangements required for synaptic plasticity. Dysregulation of Septin 8 expression or function may impair neural connectivity and contribute to neurological disorders.
Septin dysregulation has been linked to cancer and neurodegeneration. Research using SEPTIN8 antibody has revealed that abnormal expression influences cell cycle progression, apoptosis, and tumor growth. In the nervous system, altered Septin 8 activity has been associated with psychiatric and developmental disorders, reflecting its importance in synaptic regulation and cytoskeletal dynamics.
SEPTIN8 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies endogenous protein levels, immunohistochemistry highlights expression in proliferative tissues, and immunofluorescence demonstrates cytoskeletal localization. These applications make SEPTIN8 antibody a versatile reagent in cytoskeletal and neuronal research.
By supplying validated SEPTIN8 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
